ANO1 Knockout cell line (PC-3)

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location： Home > Products > KO Cell Lines > ANO1 Knockout cell line (PC-3)

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ANO1 Knockout cell line (PC-3) Order

ANO1 Knockout cell line (PC-3)
Catalog#
YKO-H1160
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
ANO1 Knockout cell line (PC-3)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
ANO1 Knockout cell line (PC-3)
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:4
Species
Human
Gene Name
ANO1
Gene ID
55107
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%F12K+10%FBS
Target region
Knockout region: Exon 2 (based on transcript ANO1-202)
Validation: PCR+Sanger Sequencing
ANO1 Knockout cell line (PC-3) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:PC-3		 STR Info (Cell bank)
Cell Line:PC-3
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 13131313
D13S317 11111111
D7S820 811811
D16S539 11111111
VWA 17171717
TH01 6767
AMEL XXXX
TPOX 8989
CSF1PO 11111111
D12S391 2121
FGA 2424
D2S1338 1820
D21S11 2931.2
D18S51 1415
D8S1179 1313
D3S1358 1616
D6S1043 1818
PENTAE 1017
D19S433 1417.2
PENTAD 99
D1S1656 1216
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the ANO1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the PC-3 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, ANO1 gene knockout ANO1 Knockout cell line (PC-3) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
ANO1 Knockout cell line (PC-3)
ANO1 Knockout cell line (PC-3)
Catalog#
YKO-H1160
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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